10 39 Search Results


pi4kiiibeta  (MedChemExpress)
93
MedChemExpress pi4kiiibeta
Effects of PI4KIIIβ inhibition and OSBP silencing on cargo sorting, trafficking, and cyst morphology. (A) MDCK cells were transfected with mCherry-OSBP-expressing plasmid and mounted for epifluorescence time-lapse microscopy the next day. PIK93 was added to the cells for 5 min at 500 nM concentration. PIK93 treatment reduced the TGN-associated OSBP pool by 75%. N = number of independent experiments presented, n = number of cells analyzed. Bar = 10 μm. (B) Additional images showing the effect of PIK93 treatment on MDCK cyst morphology. (C) MDCK cells were nucleofected with mCherry-OSBP-expressing plasmid and time-lapse fluorescence imaging was performed on the <t>next</t> <t>day.</t> <t>PI4KIIIbeta-IN-10</t> was added to the cells after 5 min at 100 nM concentration. PI4KIIIbeta-IN-10 reduced the TGN-associated Golgi pool by ∼70%. N = number of independent experiments presented, n = number of cells analyzed. Bar = 10 μm. (D and E) MDCK cells were transfected with basolateral CDH1 and apical EGFP-GPI RUSH model cargo-expressing constructs. 24 h later, biotin was added to the samples, and then cells were kept at 19.5°C for 1.5 h to synchronize cargoes at the TGN. Following this, cells were kept at 37°C for 40 min in the absence or presence of 100 nM PI4KIIIbeta-IN-10. After the treatment, cells were fixed, then confocal microscopy was performed. Line-plots indicate fluorescence intensities of each cargo at the corresponding areas and arrowheads point to post-Golgi vesicles positive for single or double cargoes. (E) Distribution of each single- and double cargo-containing post-Golgi vesicles in control and PI4KIIIbeta-IN-10-treated cells. At least 9 cells/condition were quantified; in each cell, at least 30 post-Golgi vesicles were counted. N = number of independent experiments presented, n = number of cells/sample used for the analysis. Error bars show SEM and P values were calculated by two-way ANOVA followed by Sidak’s multiple comparison test. Bar = 10 μm, diameter of the insets = 9 μm. (F and G) MDCK cells were seeded into Matrigel matrix and cysts were left to develop for 72 h in the absence or the presence of 25 nM PI4KIIIbeta-IN-10. (I) The number of cysts with luminal defects increased upon PI4KIIIbeta-IN-10 treatment. N = number of independent experiments. P value was calculated by unpaired t test. Bar = 10 μm, diameter of the insets = 14.5 μm. (H) Western blot analysis to test the efficacy of OSBP silencing following transfection with RUSH cargoes. (I) Results of three independent RUSH experiments using CDH1 and EGFP-GPI model cargoes upon OSBP silencing. Thin lines indicate individual cells. Bold lines correspond to means, while shaded areas indicate SEM. n = number of cells analyzed/experiment to obtain mean and SEM. P values were calculated by two-way ANOVA followed by Sidak’s multiple comparison test. ‡ indicates the representative experiments shown in , respectively. (J) Additional cysts to show the effect of OSBP silencing on MDCK cyst development. Bar = 10 μm. Source data are available for this figure: .
Pi4kiiibeta, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/10+39/PI4KIIIbeta-IN-10/pmc10664280-257-0-1
Average 93 stars, based on 1 article reviews
pi4kiiibeta - by Bioz Stars, 2026-10
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7 z 10 z 13 z hexadecatetraenoic acid larodan co stockholm sweden  (Larodan)
91
Larodan 7 z 10 z 13 z hexadecatetraenoic acid larodan co stockholm sweden
Effects of PI4KIIIβ inhibition and OSBP silencing on cargo sorting, trafficking, and cyst morphology. (A) MDCK cells were transfected with mCherry-OSBP-expressing plasmid and mounted for epifluorescence time-lapse microscopy the next day. PIK93 was added to the cells for 5 min at 500 nM concentration. PIK93 treatment reduced the TGN-associated OSBP pool by 75%. N = number of independent experiments presented, n = number of cells analyzed. Bar = 10 μm. (B) Additional images showing the effect of PIK93 treatment on MDCK cyst morphology. (C) MDCK cells were nucleofected with mCherry-OSBP-expressing plasmid and time-lapse fluorescence imaging was performed on the <t>next</t> <t>day.</t> <t>PI4KIIIbeta-IN-10</t> was added to the cells after 5 min at 100 nM concentration. PI4KIIIbeta-IN-10 reduced the TGN-associated Golgi pool by ∼70%. N = number of independent experiments presented, n = number of cells analyzed. Bar = 10 μm. (D and E) MDCK cells were transfected with basolateral CDH1 and apical EGFP-GPI RUSH model cargo-expressing constructs. 24 h later, biotin was added to the samples, and then cells were kept at 19.5°C for 1.5 h to synchronize cargoes at the TGN. Following this, cells were kept at 37°C for 40 min in the absence or presence of 100 nM PI4KIIIbeta-IN-10. After the treatment, cells were fixed, then confocal microscopy was performed. Line-plots indicate fluorescence intensities of each cargo at the corresponding areas and arrowheads point to post-Golgi vesicles positive for single or double cargoes. (E) Distribution of each single- and double cargo-containing post-Golgi vesicles in control and PI4KIIIbeta-IN-10-treated cells. At least 9 cells/condition were quantified; in each cell, at least 30 post-Golgi vesicles were counted. N = number of independent experiments presented, n = number of cells/sample used for the analysis. Error bars show SEM and P values were calculated by two-way ANOVA followed by Sidak’s multiple comparison test. Bar = 10 μm, diameter of the insets = 9 μm. (F and G) MDCK cells were seeded into Matrigel matrix and cysts were left to develop for 72 h in the absence or the presence of 25 nM PI4KIIIbeta-IN-10. (I) The number of cysts with luminal defects increased upon PI4KIIIbeta-IN-10 treatment. N = number of independent experiments. P value was calculated by unpaired t test. Bar = 10 μm, diameter of the insets = 14.5 μm. (H) Western blot analysis to test the efficacy of OSBP silencing following transfection with RUSH cargoes. (I) Results of three independent RUSH experiments using CDH1 and EGFP-GPI model cargoes upon OSBP silencing. Thin lines indicate individual cells. Bold lines correspond to means, while shaded areas indicate SEM. n = number of cells analyzed/experiment to obtain mean and SEM. P values were calculated by two-way ANOVA followed by Sidak’s multiple comparison test. ‡ indicates the representative experiments shown in , respectively. (J) Additional cysts to show the effect of OSBP silencing on MDCK cyst development. Bar = 10 μm. Source data are available for this figure: .
7 Z 10 Z 13 Z Hexadecatetraenoic Acid Larodan Co Stockholm Sweden, supplied by Larodan, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/10+39/4(Z)%2C7(Z)%2C10(Z)%2C13(Z)-hexadecatetraenoic+acid/pmc09457472__pnas__2202930119__sapp-115-14-16
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fmoc l ala oh  (Chem Impex International)
95
Chem Impex International fmoc l ala oh
Effects of PI4KIIIβ inhibition and OSBP silencing on cargo sorting, trafficking, and cyst morphology. (A) MDCK cells were transfected with mCherry-OSBP-expressing plasmid and mounted for epifluorescence time-lapse microscopy the next day. PIK93 was added to the cells for 5 min at 500 nM concentration. PIK93 treatment reduced the TGN-associated OSBP pool by 75%. N = number of independent experiments presented, n = number of cells analyzed. Bar = 10 μm. (B) Additional images showing the effect of PIK93 treatment on MDCK cyst morphology. (C) MDCK cells were nucleofected with mCherry-OSBP-expressing plasmid and time-lapse fluorescence imaging was performed on the <t>next</t> <t>day.</t> <t>PI4KIIIbeta-IN-10</t> was added to the cells after 5 min at 100 nM concentration. PI4KIIIbeta-IN-10 reduced the TGN-associated Golgi pool by ∼70%. N = number of independent experiments presented, n = number of cells analyzed. Bar = 10 μm. (D and E) MDCK cells were transfected with basolateral CDH1 and apical EGFP-GPI RUSH model cargo-expressing constructs. 24 h later, biotin was added to the samples, and then cells were kept at 19.5°C for 1.5 h to synchronize cargoes at the TGN. Following this, cells were kept at 37°C for 40 min in the absence or presence of 100 nM PI4KIIIbeta-IN-10. After the treatment, cells were fixed, then confocal microscopy was performed. Line-plots indicate fluorescence intensities of each cargo at the corresponding areas and arrowheads point to post-Golgi vesicles positive for single or double cargoes. (E) Distribution of each single- and double cargo-containing post-Golgi vesicles in control and PI4KIIIbeta-IN-10-treated cells. At least 9 cells/condition were quantified; in each cell, at least 30 post-Golgi vesicles were counted. N = number of independent experiments presented, n = number of cells/sample used for the analysis. Error bars show SEM and P values were calculated by two-way ANOVA followed by Sidak’s multiple comparison test. Bar = 10 μm, diameter of the insets = 9 μm. (F and G) MDCK cells were seeded into Matrigel matrix and cysts were left to develop for 72 h in the absence or the presence of 25 nM PI4KIIIbeta-IN-10. (I) The number of cysts with luminal defects increased upon PI4KIIIbeta-IN-10 treatment. N = number of independent experiments. P value was calculated by unpaired t test. Bar = 10 μm, diameter of the insets = 14.5 μm. (H) Western blot analysis to test the efficacy of OSBP silencing following transfection with RUSH cargoes. (I) Results of three independent RUSH experiments using CDH1 and EGFP-GPI model cargoes upon OSBP silencing. Thin lines indicate individual cells. Bold lines correspond to means, while shaded areas indicate SEM. n = number of cells analyzed/experiment to obtain mean and SEM. P values were calculated by two-way ANOVA followed by Sidak’s multiple comparison test. ‡ indicates the representative experiments shown in , respectively. (J) Additional cysts to show the effect of OSBP silencing on MDCK cyst development. Bar = 10 μm. Source data are available for this figure: .
Fmoc L Ala Oh, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/10+39/Fmoc-L-alanine/pm33739101__ic1c00087_si_001-46-22-32
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hydroperoxy 4z 7z 10z 13z 15e 19z docosahexaenoic acid 17 hpdhe  (Larodan)
94
Larodan hydroperoxy 4z 7z 10z 13z 15e 19z docosahexaenoic acid 17 hpdhe
Effects of PI4KIIIβ inhibition and OSBP silencing on cargo sorting, trafficking, and cyst morphology. (A) MDCK cells were transfected with mCherry-OSBP-expressing plasmid and mounted for epifluorescence time-lapse microscopy the next day. PIK93 was added to the cells for 5 min at 500 nM concentration. PIK93 treatment reduced the TGN-associated OSBP pool by 75%. N = number of independent experiments presented, n = number of cells analyzed. Bar = 10 μm. (B) Additional images showing the effect of PIK93 treatment on MDCK cyst morphology. (C) MDCK cells were nucleofected with mCherry-OSBP-expressing plasmid and time-lapse fluorescence imaging was performed on the <t>next</t> <t>day.</t> <t>PI4KIIIbeta-IN-10</t> was added to the cells after 5 min at 100 nM concentration. PI4KIIIbeta-IN-10 reduced the TGN-associated Golgi pool by ∼70%. N = number of independent experiments presented, n = number of cells analyzed. Bar = 10 μm. (D and E) MDCK cells were transfected with basolateral CDH1 and apical EGFP-GPI RUSH model cargo-expressing constructs. 24 h later, biotin was added to the samples, and then cells were kept at 19.5°C for 1.5 h to synchronize cargoes at the TGN. Following this, cells were kept at 37°C for 40 min in the absence or presence of 100 nM PI4KIIIbeta-IN-10. After the treatment, cells were fixed, then confocal microscopy was performed. Line-plots indicate fluorescence intensities of each cargo at the corresponding areas and arrowheads point to post-Golgi vesicles positive for single or double cargoes. (E) Distribution of each single- and double cargo-containing post-Golgi vesicles in control and PI4KIIIbeta-IN-10-treated cells. At least 9 cells/condition were quantified; in each cell, at least 30 post-Golgi vesicles were counted. N = number of independent experiments presented, n = number of cells/sample used for the analysis. Error bars show SEM and P values were calculated by two-way ANOVA followed by Sidak’s multiple comparison test. Bar = 10 μm, diameter of the insets = 9 μm. (F and G) MDCK cells were seeded into Matrigel matrix and cysts were left to develop for 72 h in the absence or the presence of 25 nM PI4KIIIbeta-IN-10. (I) The number of cysts with luminal defects increased upon PI4KIIIbeta-IN-10 treatment. N = number of independent experiments. P value was calculated by unpaired t test. Bar = 10 μm, diameter of the insets = 14.5 μm. (H) Western blot analysis to test the efficacy of OSBP silencing following transfection with RUSH cargoes. (I) Results of three independent RUSH experiments using CDH1 and EGFP-GPI model cargoes upon OSBP silencing. Thin lines indicate individual cells. Bold lines correspond to means, while shaded areas indicate SEM. n = number of cells analyzed/experiment to obtain mean and SEM. P values were calculated by two-way ANOVA followed by Sidak’s multiple comparison test. ‡ indicates the representative experiments shown in , respectively. (J) Additional cysts to show the effect of OSBP silencing on MDCK cyst development. Bar = 10 μm. Source data are available for this figure: .
Hydroperoxy 4z 7z 10z 13z 15e 19z Docosahexaenoic Acid 17 Hpdhe, supplied by Larodan, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/10+39/17(S)-hydroperoxy-4(E)%2C7(Z)%2C10(Z)%2C13(Z)%2C15(Z)%2C19(Z)-docosahexaenoic+acid/pm41242694-85-20-26
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resveratrol  (Chem Impex International)
95
Chem Impex International resveratrol
Effects of PI4KIIIβ inhibition and OSBP silencing on cargo sorting, trafficking, and cyst morphology. (A) MDCK cells were transfected with mCherry-OSBP-expressing plasmid and mounted for epifluorescence time-lapse microscopy the next day. PIK93 was added to the cells for 5 min at 500 nM concentration. PIK93 treatment reduced the TGN-associated OSBP pool by 75%. N = number of independent experiments presented, n = number of cells analyzed. Bar = 10 μm. (B) Additional images showing the effect of PIK93 treatment on MDCK cyst morphology. (C) MDCK cells were nucleofected with mCherry-OSBP-expressing plasmid and time-lapse fluorescence imaging was performed on the <t>next</t> <t>day.</t> <t>PI4KIIIbeta-IN-10</t> was added to the cells after 5 min at 100 nM concentration. PI4KIIIbeta-IN-10 reduced the TGN-associated Golgi pool by ∼70%. N = number of independent experiments presented, n = number of cells analyzed. Bar = 10 μm. (D and E) MDCK cells were transfected with basolateral CDH1 and apical EGFP-GPI RUSH model cargo-expressing constructs. 24 h later, biotin was added to the samples, and then cells were kept at 19.5°C for 1.5 h to synchronize cargoes at the TGN. Following this, cells were kept at 37°C for 40 min in the absence or presence of 100 nM PI4KIIIbeta-IN-10. After the treatment, cells were fixed, then confocal microscopy was performed. Line-plots indicate fluorescence intensities of each cargo at the corresponding areas and arrowheads point to post-Golgi vesicles positive for single or double cargoes. (E) Distribution of each single- and double cargo-containing post-Golgi vesicles in control and PI4KIIIbeta-IN-10-treated cells. At least 9 cells/condition were quantified; in each cell, at least 30 post-Golgi vesicles were counted. N = number of independent experiments presented, n = number of cells/sample used for the analysis. Error bars show SEM and P values were calculated by two-way ANOVA followed by Sidak’s multiple comparison test. Bar = 10 μm, diameter of the insets = 9 μm. (F and G) MDCK cells were seeded into Matrigel matrix and cysts were left to develop for 72 h in the absence or the presence of 25 nM PI4KIIIbeta-IN-10. (I) The number of cysts with luminal defects increased upon PI4KIIIbeta-IN-10 treatment. N = number of independent experiments. P value was calculated by unpaired t test. Bar = 10 μm, diameter of the insets = 14.5 μm. (H) Western blot analysis to test the efficacy of OSBP silencing following transfection with RUSH cargoes. (I) Results of three independent RUSH experiments using CDH1 and EGFP-GPI model cargoes upon OSBP silencing. Thin lines indicate individual cells. Bold lines correspond to means, while shaded areas indicate SEM. n = number of cells analyzed/experiment to obtain mean and SEM. P values were calculated by two-way ANOVA followed by Sidak’s multiple comparison test. ‡ indicates the representative experiments shown in , respectively. (J) Additional cysts to show the effect of OSBP silencing on MDCK cyst development. Bar = 10 μm. Source data are available for this figure: .
Resveratrol, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/10+39/Resveratrol/ppr0571525-47-0-8
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oxo 10 15 z phytodienoic acid opda  (Larodan)
90
Larodan oxo 10 15 z phytodienoic acid opda
Effects of PI4KIIIβ inhibition and OSBP silencing on cargo sorting, trafficking, and cyst morphology. (A) MDCK cells were transfected with mCherry-OSBP-expressing plasmid and mounted for epifluorescence time-lapse microscopy the next day. PIK93 was added to the cells for 5 min at 500 nM concentration. PIK93 treatment reduced the TGN-associated OSBP pool by 75%. N = number of independent experiments presented, n = number of cells analyzed. Bar = 10 μm. (B) Additional images showing the effect of PIK93 treatment on MDCK cyst morphology. (C) MDCK cells were nucleofected with mCherry-OSBP-expressing plasmid and time-lapse fluorescence imaging was performed on the <t>next</t> <t>day.</t> <t>PI4KIIIbeta-IN-10</t> was added to the cells after 5 min at 100 nM concentration. PI4KIIIbeta-IN-10 reduced the TGN-associated Golgi pool by ∼70%. N = number of independent experiments presented, n = number of cells analyzed. Bar = 10 μm. (D and E) MDCK cells were transfected with basolateral CDH1 and apical EGFP-GPI RUSH model cargo-expressing constructs. 24 h later, biotin was added to the samples, and then cells were kept at 19.5°C for 1.5 h to synchronize cargoes at the TGN. Following this, cells were kept at 37°C for 40 min in the absence or presence of 100 nM PI4KIIIbeta-IN-10. After the treatment, cells were fixed, then confocal microscopy was performed. Line-plots indicate fluorescence intensities of each cargo at the corresponding areas and arrowheads point to post-Golgi vesicles positive for single or double cargoes. (E) Distribution of each single- and double cargo-containing post-Golgi vesicles in control and PI4KIIIbeta-IN-10-treated cells. At least 9 cells/condition were quantified; in each cell, at least 30 post-Golgi vesicles were counted. N = number of independent experiments presented, n = number of cells/sample used for the analysis. Error bars show SEM and P values were calculated by two-way ANOVA followed by Sidak’s multiple comparison test. Bar = 10 μm, diameter of the insets = 9 μm. (F and G) MDCK cells were seeded into Matrigel matrix and cysts were left to develop for 72 h in the absence or the presence of 25 nM PI4KIIIbeta-IN-10. (I) The number of cysts with luminal defects increased upon PI4KIIIbeta-IN-10 treatment. N = number of independent experiments. P value was calculated by unpaired t test. Bar = 10 μm, diameter of the insets = 14.5 μm. (H) Western blot analysis to test the efficacy of OSBP silencing following transfection with RUSH cargoes. (I) Results of three independent RUSH experiments using CDH1 and EGFP-GPI model cargoes upon OSBP silencing. Thin lines indicate individual cells. Bold lines correspond to means, while shaded areas indicate SEM. n = number of cells analyzed/experiment to obtain mean and SEM. P values were calculated by two-way ANOVA followed by Sidak’s multiple comparison test. ‡ indicates the representative experiments shown in , respectively. (J) Additional cysts to show the effect of OSBP silencing on MDCK cyst development. Bar = 10 μm. Source data are available for this figure: .
Oxo 10 15 Z Phytodienoic Acid Opda, supplied by Larodan, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/10+39/12-Oxo-10%2C15(Z)-phytodienoicacid/pmc02755848-101-0-8
Average 90 stars, based on 1 article reviews
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jacaric acid  (Larodan)
93
Larodan jacaric acid
Effects of PI4KIIIβ inhibition and OSBP silencing on cargo sorting, trafficking, and cyst morphology. (A) MDCK cells were transfected with mCherry-OSBP-expressing plasmid and mounted for epifluorescence time-lapse microscopy the next day. PIK93 was added to the cells for 5 min at 500 nM concentration. PIK93 treatment reduced the TGN-associated OSBP pool by 75%. N = number of independent experiments presented, n = number of cells analyzed. Bar = 10 μm. (B) Additional images showing the effect of PIK93 treatment on MDCK cyst morphology. (C) MDCK cells were nucleofected with mCherry-OSBP-expressing plasmid and time-lapse fluorescence imaging was performed on the <t>next</t> <t>day.</t> <t>PI4KIIIbeta-IN-10</t> was added to the cells after 5 min at 100 nM concentration. PI4KIIIbeta-IN-10 reduced the TGN-associated Golgi pool by ∼70%. N = number of independent experiments presented, n = number of cells analyzed. Bar = 10 μm. (D and E) MDCK cells were transfected with basolateral CDH1 and apical EGFP-GPI RUSH model cargo-expressing constructs. 24 h later, biotin was added to the samples, and then cells were kept at 19.5°C for 1.5 h to synchronize cargoes at the TGN. Following this, cells were kept at 37°C for 40 min in the absence or presence of 100 nM PI4KIIIbeta-IN-10. After the treatment, cells were fixed, then confocal microscopy was performed. Line-plots indicate fluorescence intensities of each cargo at the corresponding areas and arrowheads point to post-Golgi vesicles positive for single or double cargoes. (E) Distribution of each single- and double cargo-containing post-Golgi vesicles in control and PI4KIIIbeta-IN-10-treated cells. At least 9 cells/condition were quantified; in each cell, at least 30 post-Golgi vesicles were counted. N = number of independent experiments presented, n = number of cells/sample used for the analysis. Error bars show SEM and P values were calculated by two-way ANOVA followed by Sidak’s multiple comparison test. Bar = 10 μm, diameter of the insets = 9 μm. (F and G) MDCK cells were seeded into Matrigel matrix and cysts were left to develop for 72 h in the absence or the presence of 25 nM PI4KIIIbeta-IN-10. (I) The number of cysts with luminal defects increased upon PI4KIIIbeta-IN-10 treatment. N = number of independent experiments. P value was calculated by unpaired t test. Bar = 10 μm, diameter of the insets = 14.5 μm. (H) Western blot analysis to test the efficacy of OSBP silencing following transfection with RUSH cargoes. (I) Results of three independent RUSH experiments using CDH1 and EGFP-GPI model cargoes upon OSBP silencing. Thin lines indicate individual cells. Bold lines correspond to means, while shaded areas indicate SEM. n = number of cells analyzed/experiment to obtain mean and SEM. P values were calculated by two-way ANOVA followed by Sidak’s multiple comparison test. ‡ indicates the representative experiments shown in , respectively. (J) Additional cysts to show the effect of OSBP silencing on MDCK cyst development. Bar = 10 μm. Source data are available for this figure: .
Jacaric Acid, supplied by Larodan, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/10+39/9-oxo-11-(3-pentyl-2-oxiranyl)-10(E)-undecenoic+acid/pm26629697-30-3-23
Average 93 stars, based on 1 article reviews
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trihydroxy octadec 1 2 enoic acid  (Larodan)
92
Larodan trihydroxy octadec 1 2 enoic acid
Effects of PI4KIIIβ inhibition and OSBP silencing on cargo sorting, trafficking, and cyst morphology. (A) MDCK cells were transfected with mCherry-OSBP-expressing plasmid and mounted for epifluorescence time-lapse microscopy the next day. PIK93 was added to the cells for 5 min at 500 nM concentration. PIK93 treatment reduced the TGN-associated OSBP pool by 75%. N = number of independent experiments presented, n = number of cells analyzed. Bar = 10 μm. (B) Additional images showing the effect of PIK93 treatment on MDCK cyst morphology. (C) MDCK cells were nucleofected with mCherry-OSBP-expressing plasmid and time-lapse fluorescence imaging was performed on the <t>next</t> <t>day.</t> <t>PI4KIIIbeta-IN-10</t> was added to the cells after 5 min at 100 nM concentration. PI4KIIIbeta-IN-10 reduced the TGN-associated Golgi pool by ∼70%. N = number of independent experiments presented, n = number of cells analyzed. Bar = 10 μm. (D and E) MDCK cells were transfected with basolateral CDH1 and apical EGFP-GPI RUSH model cargo-expressing constructs. 24 h later, biotin was added to the samples, and then cells were kept at 19.5°C for 1.5 h to synchronize cargoes at the TGN. Following this, cells were kept at 37°C for 40 min in the absence or presence of 100 nM PI4KIIIbeta-IN-10. After the treatment, cells were fixed, then confocal microscopy was performed. Line-plots indicate fluorescence intensities of each cargo at the corresponding areas and arrowheads point to post-Golgi vesicles positive for single or double cargoes. (E) Distribution of each single- and double cargo-containing post-Golgi vesicles in control and PI4KIIIbeta-IN-10-treated cells. At least 9 cells/condition were quantified; in each cell, at least 30 post-Golgi vesicles were counted. N = number of independent experiments presented, n = number of cells/sample used for the analysis. Error bars show SEM and P values were calculated by two-way ANOVA followed by Sidak’s multiple comparison test. Bar = 10 μm, diameter of the insets = 9 μm. (F and G) MDCK cells were seeded into Matrigel matrix and cysts were left to develop for 72 h in the absence or the presence of 25 nM PI4KIIIbeta-IN-10. (I) The number of cysts with luminal defects increased upon PI4KIIIbeta-IN-10 treatment. N = number of independent experiments. P value was calculated by unpaired t test. Bar = 10 μm, diameter of the insets = 14.5 μm. (H) Western blot analysis to test the efficacy of OSBP silencing following transfection with RUSH cargoes. (I) Results of three independent RUSH experiments using CDH1 and EGFP-GPI model cargoes upon OSBP silencing. Thin lines indicate individual cells. Bold lines correspond to means, while shaded areas indicate SEM. n = number of cells analyzed/experiment to obtain mean and SEM. P values were calculated by two-way ANOVA followed by Sidak’s multiple comparison test. ‡ indicates the representative experiments shown in , respectively. (J) Additional cysts to show the effect of OSBP silencing on MDCK cyst development. Bar = 10 μm. Source data are available for this figure: .
Trihydroxy Octadec 1 2 Enoic Acid, supplied by Larodan, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/10+39/9(S)%2C10(S)%2C11(R)-Trihydroxy-12(Z)%2C15(Z)-octadecadienoic+acid/pmc12814519-87-42-49
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amphotericin b  (Chem Impex International)
95
Chem Impex International amphotericin b
Effects of PI4KIIIβ inhibition and OSBP silencing on cargo sorting, trafficking, and cyst morphology. (A) MDCK cells were transfected with mCherry-OSBP-expressing plasmid and mounted for epifluorescence time-lapse microscopy the next day. PIK93 was added to the cells for 5 min at 500 nM concentration. PIK93 treatment reduced the TGN-associated OSBP pool by 75%. N = number of independent experiments presented, n = number of cells analyzed. Bar = 10 μm. (B) Additional images showing the effect of PIK93 treatment on MDCK cyst morphology. (C) MDCK cells were nucleofected with mCherry-OSBP-expressing plasmid and time-lapse fluorescence imaging was performed on the <t>next</t> <t>day.</t> <t>PI4KIIIbeta-IN-10</t> was added to the cells after 5 min at 100 nM concentration. PI4KIIIbeta-IN-10 reduced the TGN-associated Golgi pool by ∼70%. N = number of independent experiments presented, n = number of cells analyzed. Bar = 10 μm. (D and E) MDCK cells were transfected with basolateral CDH1 and apical EGFP-GPI RUSH model cargo-expressing constructs. 24 h later, biotin was added to the samples, and then cells were kept at 19.5°C for 1.5 h to synchronize cargoes at the TGN. Following this, cells were kept at 37°C for 40 min in the absence or presence of 100 nM PI4KIIIbeta-IN-10. After the treatment, cells were fixed, then confocal microscopy was performed. Line-plots indicate fluorescence intensities of each cargo at the corresponding areas and arrowheads point to post-Golgi vesicles positive for single or double cargoes. (E) Distribution of each single- and double cargo-containing post-Golgi vesicles in control and PI4KIIIbeta-IN-10-treated cells. At least 9 cells/condition were quantified; in each cell, at least 30 post-Golgi vesicles were counted. N = number of independent experiments presented, n = number of cells/sample used for the analysis. Error bars show SEM and P values were calculated by two-way ANOVA followed by Sidak’s multiple comparison test. Bar = 10 μm, diameter of the insets = 9 μm. (F and G) MDCK cells were seeded into Matrigel matrix and cysts were left to develop for 72 h in the absence or the presence of 25 nM PI4KIIIbeta-IN-10. (I) The number of cysts with luminal defects increased upon PI4KIIIbeta-IN-10 treatment. N = number of independent experiments. P value was calculated by unpaired t test. Bar = 10 μm, diameter of the insets = 14.5 μm. (H) Western blot analysis to test the efficacy of OSBP silencing following transfection with RUSH cargoes. (I) Results of three independent RUSH experiments using CDH1 and EGFP-GPI model cargoes upon OSBP silencing. Thin lines indicate individual cells. Bold lines correspond to means, while shaded areas indicate SEM. n = number of cells analyzed/experiment to obtain mean and SEM. P values were calculated by two-way ANOVA followed by Sidak’s multiple comparison test. ‡ indicates the representative experiments shown in , respectively. (J) Additional cysts to show the effect of OSBP silencing on MDCK cyst development. Bar = 10 μm. Source data are available for this figure: .
Amphotericin B, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hydroperoxy 5z 8 z 10 e 14 z 17 zeicosapentaenoic acid 12 hpepe  (Larodan)
93
Larodan hydroperoxy 5z 8 z 10 e 14 z 17 zeicosapentaenoic acid 12 hpepe
Fig. 5 Standard curves of different hydroperoxides measured by the FOX assay using 440 mM of perchloric acid. CuHP: cumene hydroperoxide, HP: hydrogen peroxide, 13-HPODE: 13(S)- hydroperoxy-9Z,11E-octadecadienoic acid, 13-HPOTE: 13(S)- hydroperoxy-9Z,11E,15Z-octadecatrienoic acid, 15-HPETE: 15(S)- hydroperoxy-5Z,8Z,11Z,13E-eicosatetraenoic acid, <t>12-HPEPE:</t> <t>12(S)-hydroperoxy-5Z,8Z,10E,14Z,17Z-eicosapentaenoic</t> acid, and 17-HPDHE: 17(S)-hydroperoxy-4Z,7Z,10Z,13Z,15E,19Z-docosahex- aenoic acid. Error bars represent mean ± SD (n = 3). When not vis- ible, the error bars are hidden below the markers
Hydroperoxy 5z 8 Z 10 E 14 Z 17 Zeicosapentaenoic Acid 12 Hpepe, supplied by Larodan, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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2s 2 methyl 4  (Enamine Ltd)
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Enamine Ltd 2s 2 methyl 4
Fig. 5 Standard curves of different hydroperoxides measured by the FOX assay using 440 mM of perchloric acid. CuHP: cumene hydroperoxide, HP: hydrogen peroxide, 13-HPODE: 13(S)- hydroperoxy-9Z,11E-octadecadienoic acid, 13-HPOTE: 13(S)- hydroperoxy-9Z,11E,15Z-octadecatrienoic acid, 15-HPETE: 15(S)- hydroperoxy-5Z,8Z,11Z,13E-eicosatetraenoic acid, <t>12-HPEPE:</t> <t>12(S)-hydroperoxy-5Z,8Z,10E,14Z,17Z-eicosapentaenoic</t> acid, and 17-HPDHE: 17(S)-hydroperoxy-4Z,7Z,10Z,13Z,15E,19Z-docosahex- aenoic acid. Error bars represent mean ± SD (n = 3). When not vis- ible, the error bars are hidden below the markers
2s 2 Methyl 4, supplied by Enamine Ltd, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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7z  (Larodan)
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Larodan 7z
Fig. 5 Standard curves of different hydroperoxides measured by the FOX assay using 440 mM of perchloric acid. CuHP: cumene hydroperoxide, HP: hydrogen peroxide, 13-HPODE: 13(S)- hydroperoxy-9Z,11E-octadecadienoic acid, 13-HPOTE: 13(S)- hydroperoxy-9Z,11E,15Z-octadecatrienoic acid, 15-HPETE: 15(S)- hydroperoxy-5Z,8Z,11Z,13E-eicosatetraenoic acid, <t>12-HPEPE:</t> <t>12(S)-hydroperoxy-5Z,8Z,10E,14Z,17Z-eicosapentaenoic</t> acid, and 17-HPDHE: 17(S)-hydroperoxy-4Z,7Z,10Z,13Z,15E,19Z-docosahex- aenoic acid. Error bars represent mean ± SD (n = 3). When not vis- ible, the error bars are hidden below the markers
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Image Search Results


Effects of PI4KIIIβ inhibition and OSBP silencing on cargo sorting, trafficking, and cyst morphology. (A) MDCK cells were transfected with mCherry-OSBP-expressing plasmid and mounted for epifluorescence time-lapse microscopy the next day. PIK93 was added to the cells for 5 min at 500 nM concentration. PIK93 treatment reduced the TGN-associated OSBP pool by 75%. N = number of independent experiments presented, n = number of cells analyzed. Bar = 10 μm. (B) Additional images showing the effect of PIK93 treatment on MDCK cyst morphology. (C) MDCK cells were nucleofected with mCherry-OSBP-expressing plasmid and time-lapse fluorescence imaging was performed on the next day. PI4KIIIbeta-IN-10 was added to the cells after 5 min at 100 nM concentration. PI4KIIIbeta-IN-10 reduced the TGN-associated Golgi pool by ∼70%. N = number of independent experiments presented, n = number of cells analyzed. Bar = 10 μm. (D and E) MDCK cells were transfected with basolateral CDH1 and apical EGFP-GPI RUSH model cargo-expressing constructs. 24 h later, biotin was added to the samples, and then cells were kept at 19.5°C for 1.5 h to synchronize cargoes at the TGN. Following this, cells were kept at 37°C for 40 min in the absence or presence of 100 nM PI4KIIIbeta-IN-10. After the treatment, cells were fixed, then confocal microscopy was performed. Line-plots indicate fluorescence intensities of each cargo at the corresponding areas and arrowheads point to post-Golgi vesicles positive for single or double cargoes. (E) Distribution of each single- and double cargo-containing post-Golgi vesicles in control and PI4KIIIbeta-IN-10-treated cells. At least 9 cells/condition were quantified; in each cell, at least 30 post-Golgi vesicles were counted. N = number of independent experiments presented, n = number of cells/sample used for the analysis. Error bars show SEM and P values were calculated by two-way ANOVA followed by Sidak’s multiple comparison test. Bar = 10 μm, diameter of the insets = 9 μm. (F and G) MDCK cells were seeded into Matrigel matrix and cysts were left to develop for 72 h in the absence or the presence of 25 nM PI4KIIIbeta-IN-10. (I) The number of cysts with luminal defects increased upon PI4KIIIbeta-IN-10 treatment. N = number of independent experiments. P value was calculated by unpaired t test. Bar = 10 μm, diameter of the insets = 14.5 μm. (H) Western blot analysis to test the efficacy of OSBP silencing following transfection with RUSH cargoes. (I) Results of three independent RUSH experiments using CDH1 and EGFP-GPI model cargoes upon OSBP silencing. Thin lines indicate individual cells. Bold lines correspond to means, while shaded areas indicate SEM. n = number of cells analyzed/experiment to obtain mean and SEM. P values were calculated by two-way ANOVA followed by Sidak’s multiple comparison test. ‡ indicates the representative experiments shown in , respectively. (J) Additional cysts to show the effect of OSBP silencing on MDCK cyst development. Bar = 10 μm. Source data are available for this figure: .

Journal: The Journal of Cell Biology

Article Title: Lipid exchange at ER–trans-Golgi contact sites governs polarized cargo sorting

doi: 10.1083/jcb.202307051

Figure Lengend Snippet: Effects of PI4KIIIβ inhibition and OSBP silencing on cargo sorting, trafficking, and cyst morphology. (A) MDCK cells were transfected with mCherry-OSBP-expressing plasmid and mounted for epifluorescence time-lapse microscopy the next day. PIK93 was added to the cells for 5 min at 500 nM concentration. PIK93 treatment reduced the TGN-associated OSBP pool by 75%. N = number of independent experiments presented, n = number of cells analyzed. Bar = 10 μm. (B) Additional images showing the effect of PIK93 treatment on MDCK cyst morphology. (C) MDCK cells were nucleofected with mCherry-OSBP-expressing plasmid and time-lapse fluorescence imaging was performed on the next day. PI4KIIIbeta-IN-10 was added to the cells after 5 min at 100 nM concentration. PI4KIIIbeta-IN-10 reduced the TGN-associated Golgi pool by ∼70%. N = number of independent experiments presented, n = number of cells analyzed. Bar = 10 μm. (D and E) MDCK cells were transfected with basolateral CDH1 and apical EGFP-GPI RUSH model cargo-expressing constructs. 24 h later, biotin was added to the samples, and then cells were kept at 19.5°C for 1.5 h to synchronize cargoes at the TGN. Following this, cells were kept at 37°C for 40 min in the absence or presence of 100 nM PI4KIIIbeta-IN-10. After the treatment, cells were fixed, then confocal microscopy was performed. Line-plots indicate fluorescence intensities of each cargo at the corresponding areas and arrowheads point to post-Golgi vesicles positive for single or double cargoes. (E) Distribution of each single- and double cargo-containing post-Golgi vesicles in control and PI4KIIIbeta-IN-10-treated cells. At least 9 cells/condition were quantified; in each cell, at least 30 post-Golgi vesicles were counted. N = number of independent experiments presented, n = number of cells/sample used for the analysis. Error bars show SEM and P values were calculated by two-way ANOVA followed by Sidak’s multiple comparison test. Bar = 10 μm, diameter of the insets = 9 μm. (F and G) MDCK cells were seeded into Matrigel matrix and cysts were left to develop for 72 h in the absence or the presence of 25 nM PI4KIIIbeta-IN-10. (I) The number of cysts with luminal defects increased upon PI4KIIIbeta-IN-10 treatment. N = number of independent experiments. P value was calculated by unpaired t test. Bar = 10 μm, diameter of the insets = 14.5 μm. (H) Western blot analysis to test the efficacy of OSBP silencing following transfection with RUSH cargoes. (I) Results of three independent RUSH experiments using CDH1 and EGFP-GPI model cargoes upon OSBP silencing. Thin lines indicate individual cells. Bold lines correspond to means, while shaded areas indicate SEM. n = number of cells analyzed/experiment to obtain mean and SEM. P values were calculated by two-way ANOVA followed by Sidak’s multiple comparison test. ‡ indicates the representative experiments shown in , respectively. (J) Additional cysts to show the effect of OSBP silencing on MDCK cyst development. Bar = 10 μm. Source data are available for this figure: .

Article Snippet: PI4KIIIbeta-IN-10 (MedChemExpress) was used at 100 nM for the experiments shown in , and at 25 nM for the experiments shown in .

Techniques: Inhibition, Transfection, Expressing, Plasmid Preparation, Time-lapse Microscopy, Concentration Assay, Fluorescence, Imaging, Construct, Confocal Microscopy, Control, Comparison, Western Blot

Fig. 5 Standard curves of different hydroperoxides measured by the FOX assay using 440 mM of perchloric acid. CuHP: cumene hydroperoxide, HP: hydrogen peroxide, 13-HPODE: 13(S)- hydroperoxy-9Z,11E-octadecadienoic acid, 13-HPOTE: 13(S)- hydroperoxy-9Z,11E,15Z-octadecatrienoic acid, 15-HPETE: 15(S)- hydroperoxy-5Z,8Z,11Z,13E-eicosatetraenoic acid, 12-HPEPE: 12(S)-hydroperoxy-5Z,8Z,10E,14Z,17Z-eicosapentaenoic acid, and 17-HPDHE: 17(S)-hydroperoxy-4Z,7Z,10Z,13Z,15E,19Z-docosahex- aenoic acid. Error bars represent mean ± SD (n = 3). When not vis- ible, the error bars are hidden below the markers

Journal: Applied microbiology and biotechnology

Article Title: Versatile ferrous oxidation-xylenol orange assay for high-throughput screening of lipoxygenase activity.

doi: 10.1007/s00253-024-13095-5

Figure Lengend Snippet: Fig. 5 Standard curves of different hydroperoxides measured by the FOX assay using 440 mM of perchloric acid. CuHP: cumene hydroperoxide, HP: hydrogen peroxide, 13-HPODE: 13(S)- hydroperoxy-9Z,11E-octadecadienoic acid, 13-HPOTE: 13(S)- hydroperoxy-9Z,11E,15Z-octadecatrienoic acid, 15-HPETE: 15(S)- hydroperoxy-5Z,8Z,11Z,13E-eicosatetraenoic acid, 12-HPEPE: 12(S)-hydroperoxy-5Z,8Z,10E,14Z,17Z-eicosapentaenoic acid, and 17-HPDHE: 17(S)-hydroperoxy-4Z,7Z,10Z,13Z,15E,19Z-docosahex- aenoic acid. Error bars represent mean ± SD (n = 3). When not vis- ible, the error bars are hidden below the markers

Article Snippet: Chemicals used for enzymatic reactions and FOX assay were obtained from the following sources: linoleic acid (LA; C18:2 Δ9Z,12Z) from Nu-Chek Prep, Inc., Minnesota, USA; fatty acid hydroperoxide standards (13(S)-hydroperoxy9Z,11E-octadecadienoic acid (13-HPODE), 13(S)-hydroperoxy-9Z,11E,15Z-octadecatrienoic acid (13-HPOTE), 15(S)-hydroperoxy-5Z,8Z,11Z,13E-eicosatetraenoic acid (15-HPETE), 12(S)-hydroperoxy-5Z,8Z,10E,14Z,17Zeicosapentaenoic acid (12-HPEPE), and 17(S)-hydroperoxy-4Z,7Z,10Z,13Z,15E,19Z-docosahexaenoic acid (17-HPDHE)) from Larodan, Solna, Sweden; xylenol orange tetrasodium salt, iron(II) sulfate heptahydrate, cumene hydroperoxide (CuHP), hydrogen peroxide (HP), and perchloric acid from Sigma-Aldrich, Missouri, USA.

Techniques: FOX Assay